Predictive value of sperm deoxyribonucleic acid (DNA) fragmentation index in male infertility

Predictive value of sperm deoxyribonucleic acid (DNA) fragmentation index in male infertility

BACKGROUND Recently, damage to the sperm DNA has been studied as it is associated with reduced fertilization rates, embryo quality, and pregnancy rates, also higher rates of spontaneous miscarriage. OBJECTIVE To develop a diagnostic method in predicting male infertility. MATERIAL AND METHODS The design of this study is cross-sectional. Data were retrieved from medical records of Yasmin IVF ...

Implications of ejaculatory sperm DNA fragmentation on male infertility management

Clinical aspects of sperm DNA fragmentation detection and male infertility.

Over the past 25 years, various methods have been developed to measure sperm DNA strand breaks in situ. Currently, there are four major tests of sperm DNA fragmentation, including the Comet, Tunel, sperm chromatin structure assay (SCSA) and the acridine orange test (AOT). The Comet assay is a light microscope technique where the sperm cells are mixed with melted agarose and then placed on a gla...

Insights on the predictive accuracy of the sperm DNA fragmentation tests on male infertility

tau.amegroups.com © Translational Andrology and Urology. All rights reserved. Dr. El-Sakka in his commentary on the “Clinical utility of sperm DNA fragmentation testing: practice recommendations based on clinical scenarios” by Agarwal et al. (1), has generally acknowledged the importance of clinical scenarios in remedying the current sperm DNA fragmentation (SDF) debate and identifying the prop...

Significant decrease in sperm deoxyribonucleic acid fragmentation after varicocelectomy.

OBJECTIVE To measure sperm DNA integrity values before and after varicocelectomy in patients with elevated preoperative levels of sperm DNA fragmentation. DESIGN Retrospective. SETTING Private urology clinic. PATIENT(S) Eleven patients with grade 1, 2, or 3 varicocele. INTERVENTION(S) Varicocelectomy. MAIN OUTCOME MEASURE(S) Sperm DNA fragmentation values were assessed before and afte...